Regulatory

Part:BBa_K1163101:Design

Designed by: Emiel van der Kouwe   Group: iGEM13_Evry   (2013-09-22)
Revision as of 23:53, 4 October 2013 by Eccho (Talk | contribs) (→‎References)

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pAceB promoter region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We extracted the 300 bp sequence upstream of the AceB gene in Escherichia coli. It contains a putative FUR binding site and a putative RBS.

Source

Genomic DNA.

References

Functional Interactions between the Carbon and Iron Utilization Regulators, Crp and Fur, in Escherichia coli. J. Bacteriol. February 2005 vol. 187 no. 3 980-990.