Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Tag
ccdB_Ind

Part:BBa_K1152007:Design

Designed by: Joshua Sachs   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 23:25, 4 October 2013 by Joshua (Talk | contribs)

Helper construct for NRP-Indigoidine-tagging


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3061
    Illegal BamHI site found at 891
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 341
    Illegal SapI.rc site found at 4324


Design Notes

BBa_K1152007 was assembled via Gibson Cloning out of four fragments. The modules that were required for the NRPS were amplified from B. parabrevis and P. luminescens. There were no illegal restiction sites. However in indC, the gene that encodes for the Indigoidine synthetase, two illegal restriction sites (one for EcoRI, one for SpeI) had to be removed by mutagenesis.


Source

X

References