Device

Part:BBa_K1150040

Designed by: Ilona Blank   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 23:16, 4 October 2013 by AnneM (Talk | contribs) (→‎Usage and Biology)

uniCAS RNAimer to Bla Target 2


RNaimer (Bla target 2)
Function Targeting VEGF2 with Cas9
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Usage and Biology

This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 2 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 2 target.


Freiburg 2013 used this plasmid to test the efficiency of target Bla2 by activating or repressing a SEAP reporter gene.


[edit]
Categories
Parameters
None