Part:BBa_K1123004
1G70 Protein Production Construct
This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the 1G70 Protein sequence. The FNR promotor sequence enables us to bring the 1G70 protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The 1G70 protein is part of a HIV protein which we are expressing with the sole purpose of using it as a CEST contrast agent.
TU-Eindhoven Sequencing Results
This construct has been submitted to the iGEM registry and has been privately sequenced by the iGEM 2013 TU-Eindhoven. The sequencing results were positive indicating that the sequence and part submitted to the registry are correct. Sequencing was performed by BaseClear Netherlands
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 200
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 39
Illegal XhoI site found at 611 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 269
Illegal NgoMIV site found at 353
Illegal NgoMIV site found at 437
Illegal NgoMIV site found at 521 - 1000COMPATIBLE WITH RFC[1000]
Characterization
This part is a composite part which is comprised of a number of other important parts namely BBa_K1123000 and BBa_K1123016.
This part has, in itself not been tested by us here at the TU-Eindhoven during the 2013 iGEM competition. However, as the other, important parts which make up this part have been tested, shown to work, and characterized we would like to refer you to these parts for a complete overview of the characterization of this part.
We do not think there will be a change in the functionality of any of the separate parts when combining them. A similar composite part BBa_K1123005 was combined and tested and showed no change in functionality other than a decrease in expression.
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