Reporter
Part:BBa_K1041004
Designed by: Lucy Clark Group: iGEM13_NRP-UEA-Norwich (2013-10-02)
AntG Promoter + Gus gene
Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 498
Illegal NgoMIV site found at 630
Illegal NgoMIV site found at 1227 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 896
[edit]
Categories
Parameters
None |