![](https://parts.igem.org/images/partbypart/icon_measurement.png)
Measurement
Part:BBa_K1055001
Designed by: Kai Fenzl Group: iGEM13_TU_Darmstadt (2013-09-18)
LssmOrange is a fluorophor derived from dsRed
LssmOrange is a fluorophor derived from dsRed. It features the excitation maxima at 437 nm and the emission maxima at 571 nm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 570
- 1000COMPATIBLE WITH RFC[1000]
Characterization LssmOrange
This biobrick did measure up to our expectations as shown in the following data. We also used this promoter to express our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.(E. Coli membrane anchor protein). AraC-Pbad shows a low background activity and a good respose to the induction with Arabinose.
File:Spectra LssmOrange.png
Figure 1. Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression. Induced at time=0h.
File:LssmOrange bac.png
Figure 1. Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression. Induced at time=0h.
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