Coding

Part:BBa_K1152000

Designed by: Tania Christiansen   Group: iGEM13_Heidelberg   (2013-09-16)
Revision as of 20:33, 4 October 2013 by Ladlung (Talk | contribs)

TycA_deltaCom Module from Tyrocidine

This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus parabrevis. It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine. This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of the A- (adenylation), PCP- (peptide-carrier protein) and the E- (epimerization) domain. After tycA follows the tycB gene with three modules.

This biobrick can be used for the design of different NRP synthetases. It was used in the synthetic Tripeptide I of our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518


[edit]
Categories
//cds/biosynthesis
Parameters
biology