Coding

Part:BBa_K1152003:Design

Designed by: Tania Christiansen   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 18:16, 4 October 2013 by TaniaChristiansen (Talk | contribs) (→‎Design Notes)

TycC6 Module from Tyrocidine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 48
    Illegal SapI.rc site found at 1165
    Illegal SapI.rc site found at 1402


Design Notes

This is just the coding sequence. We sequenced with VR and VF2 primer, no mutations were found (http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip ). You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new non ribosomal-peptide. http://2013.igem.org/Team:Heidelberg/Parts

Source

Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835

References