Reporter

Part:BBa_K1028002:Design

Designed by: Joseph Beton   Group: iGEM13_Leeds   (2013-09-19)
Revision as of 18:07, 4 October 2013 by Joebeton (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

A cell membrane stress reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 728


Design Notes

The parts were assembled using PCR assembly to avoid introducing a scar site between the promoter and the ribosome binding site included in part BBa_K081012 and in assembled parallel

This part uses a strong RBS to maximize fluorescence response following activation of pCpxR.


Source

The parts for this were aquired from the iGEM 2013 delivery plates.

References