Part:BBa_K1111012

Ice Nucleation Protein fused to Streptavidin Alive

Introduction

It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein found in the genome of Pseudomonas syringae, that is recognized by the E.coli protein secretion machinery, and thus also exported in that organism.

Figure 1: outline of the expected outcome of the plasmid

Usage and Biology

This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin affinity (attachment of cargo is one example, but we could also think of purification etc...).

Datasheet

For further Characterization, refer to the datasheet below.

CharacterizationSheet Page 1

CharacterizationSheet Page 2

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1727
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1649
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1727
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1727
  Illegal NgoMIV site found at 1036
  Illegal AgeI site found at 1691
  Illegal AgeI site found at 1742
  
• 1000
  COMPATIBLE WITH RFC[1000]