Coding

Part:BBa_K1033103

Designed by: Magnus Bergman, Nils Anlind, Alexander Wirtanen, Pontus Danielsson, Main Borg, Thorsteinn Olaufsson, Christoffer Frisk, Niclas Sandberg   Group: iGEM13_Uppsala   (2013-09-04)
Revision as of 17:34, 4 October 2013 by Magnus (Talk | contribs)

_NOTOC__ geranyl diphosphate synthase

Geranyl diphosphate syntease (IspA)

Catalyses the formation of dimethylallyl diphophate + isopentenyl diphosphate to geranyl diphosphate. As this is an often rate-limiting step in production of isoprenoids, it can desirable to introduce additional copies of the gene to increase yield.

This part was cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034 and biobrick restriction site.


Results

We were unsuccessful in assembling any promoter to the construct, and as such could not further characterize the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 182
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None