Regulatory

Part:BBa_K1033225:Design

Designed by: Stephanie Herman, Alona Nyberg   Group: iGEM13_Uppsala   (2013-08-23)
Revision as of 17:20, 4 October 2013 by StephyHe (Talk | contribs) (→‎Design Notes)

Promoter CP44


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was based on a consensus sequence found in Lactococcus lactis. It was synthezised by using primers containing a overhang with the standard prefix and suffix.

Source

This part is a synthetic promoter based on a promoter library from the article "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." by Peter Ruhdal Jensen and Karin Hammer.

References

P. Jensen, K Hammer Appl: The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters Environ Microbiol. 64.1 (1998) 82–87