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Regulatory

Part:BBa_K1045011:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 16:58, 4 October 2013 by Kati (Talk | contribs) (→‎Design Notes)


Promoter reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To construct this part, hybridization oligos were used. These hybridization oligos encoded the sequence of BBa_J23117 and 54 additional bases upstream of this promoter.

Source

To be continued

References