Coding

Part:BBa_K1123019

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-08-31)
Revision as of 16:38, 4 October 2013 by ArdjanvanderLinden (Talk | contribs)

Poly(Arginine-Serine) Protein

This part contains the DNA sequence of a protein of our own design. We first repeated the Arginine-Serine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added. The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI. The protein can be placed behind a promoter of your choice and expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.


[edit]
Categories
Parameters
None