Composite

Part:BBa_K1022122:Design

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-20)
Revision as of 15:47, 4 October 2013 by MaithiliKrishnan (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II : RBS TetR :TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 2317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BglII site found at 2441
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1405
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2626
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Biobrick Bba_K1022106 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119

Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft]



Source

Clonned

References