Composite

Part:BBa_K1111005:Design

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 13:40, 4 October 2013 by GigiGoesGugu (Talk | contribs) (→‎References)

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Constitutive promoter inducing GFP expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

Because the promoter is very short (35bp) we designed it's primers with overhangs that were complementary to the pBS1C3 plasmid. This allowed us to insert the promoter into the plasmid by Gibson Assembly.


Source

BioBrick BBa_J23119, designed be the 2006 berkley team BioBrick BBa_J23119

References

https://parts.igem.org/Part:BBa_J23119