Part:BBa_K1155000

Pndh*

promoter region containing an operator and a promoter controlled by FNR that allow the repression of the downstream gene in anaerobic conditions

In Escherichia coli, the switch between aerobic and anaerobic metabolism is partly regulated the transcriptional regulator FNR. This protein has a dual function: it activates genes involved in anaerobic metabolism and represses genes involved in aerobic metabolism. FNR expression is constitutive, but its activity is directly affected by the presence of oxygen which oxidizes of an essential [4Fe-4S] cluster. The expression of the ndh gene, encoding an non-proton-translocating NADH dehydrogenase is repressed by FNR in anaerobic conditions. FNR binds at two sites in the promoter region of ndh: a high affinity site (FNRI), and a low affinity, imperfect site (FNRII). In 1997, Meng and colleagues designed a modified ndh promoter region, called Pndh*, with a FNRII site engineered to have a perfect FNR binding site, improving 10 fold the degree of anaerobic repression in vivo.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]