PBAD SPL

Revision as of 10:30, 4 October 2013 by KrDa (Talk | contribs)

Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.

List of sequences:

DTU pBAD SPL

Identifier Sequencea Induced Strengthb Leakynessb
Col. 2 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA 14.2 14.2
Col. 3 CTGACGTAATCATACCGCCGAAAGTATTATCATTA 16.6 14.2
Col. 4 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA 14.2 14.2
BBa_J23102 ttgacagctagctcagtcctaggtactgtgctagc 0.86
BBa_J23103 ctgatagctagctcagtcctagggattatgctagc 0.01
BBa_J23104 ttgacagctagctcagtcctaggtattgtgctagc 0.72
BBa_J23105 tttacggctagctcagtcctaggtactatgctagc 0.24
BBa_J23106 tttacggctagctcagtcctaggtatagtgctagc 0.47
BBa_J23107 tttacggctagctcagccctaggtattatgctagc 0.36
BBa_J23108 ctgacagctagctcagtcctaggtataatgctagc 0.51
BBa_J23109 tttacagctagctcagtcctagggactgtgctagc 0.04
BBa_J23110 tttacggctagctcagtcctaggtacaatgctagc 0.33
BBa_J23111 ttgacggctagctcagtcctaggtatagtgctagc 0.58
BBa_J23112 ctgatagctagctcagtcctagggattatgctagc 0.00
BBa_J23113 ctgatggctagctcagtcctagggattatgctagc 0.01
BBa_J23114 tttatggctagctcagtcctaggtacaatgctagc 0.10
BBa_J23115 tttatagctagctcagcccttggtacaatgctagc 0.15
BBa_J23116 ttgacagctagctcagtcctagggactatgctagc 0.16
BBa_J23117 ttgacagctagctcagtcctagggattgtgctagc 0.06
BBa_J23118 ttgacggctagctcagtcctaggtattgtgctagc 0.56





Mutant name Sequence
Mut. 1 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA
Mut. 2 CTGACGTAATCATACCGCCGAAAGTATTATCATTA
Mut. 3 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA