Composite

Part:BBa_K1065305:Design

Designed by: Bruno Aor   Group: iGEM13_UNITN-Trento   (2013-09-09)
Revision as of 09:09, 4 October 2013 by Cridelbianco (Talk | contribs) (→‎References)

Blue light sensor without inverter for the production of amilGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 276
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311
    Illegal BsaI.rc site found at 175


Design Notes

Part BBa_K952003 was missing an RBS before the amilGFP CDS, thus it doesn't work. We successfully inserted the RBS by mutagenesis. For characterization we created the part BBa_K1065302 that is the same device under the control of pLac.

Source

The part consist of:
BBa_K952003: the entire blue light sensing device with fluorescent output.
RBS sequence

References

  1. Diversity and evolution of coral fluorescent proteins
    Alieva, N. O., et al. 2008. Plos One 3: e2680l.
    1. 2008. . PLoS One 3:e2680.
    2. Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
    3. Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542