Part:BBa_K1033259
Toxin-antitoxin system from lactobacillus plantarum
This is a toxin-antitoxin system originating from plasmid p256 from lactobacillus plantarum strain NC7. It has been created without the natural promotor to allow for customisable strength of the system. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been proven very efficient in previous studies. [1]
[1] Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 71
Illegal SpeI site found at 447 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 71
Illegal SpeI site found at 447 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 71
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 71
Illegal SpeI site found at 447 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 71
Illegal SpeI site found at 447 - 1000COMPATIBLE WITH RFC[1000]
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