Part:BBa_K1075021

E. coli ssrA(DAS+4)-TT

construction

The part contains the mutated E. coli(ec)ssrA (DAS+4) tag and a double terminator.

biology

The ecssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor protein ecSspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA (DAS+4) and increases the dependance of ecSspB.[http://www.ncbi.nlm.nih.gov/pubmed/16762842]

The double terminator stops the transcription at this point.

application

The ecssrA(DAS+4) tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 40
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]