Part:BBa_K1141002
Plac-RBS-KillerRed (IPTG-inducible)
KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI). So the production of KillerRed is IPTG dependent. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013.
Note that the information characterising this part was made with BBa_K1141001, which has the same sequence except for an additional Eco RI site between the Promoter and RBS. The vector for BBa_K1141001 is a high copy plasmid like pSB1C3 with ac col1 as duplication origin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 133
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 289
Illegal BsaI.rc site found at 580
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