Intermediate

Part:BBa_K1075021

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)
Revision as of 16:21, 3 October 2013 by AnnikaS (Talk | contribs)

E. coli ssrA(DAS+4)-TT

ecssrA(DAS+4)-TT

construction

The part contains the mutated ssrA (DAS+4) tag and a double terminator.


biology

The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ssrA (DAS+4) weakens a direct binding between proteases and ssrA and increases the dependance of sspB.

The double terminator stops the transcription at this point.


application

As we want to control protein degradation by controlling the function of SspB, we tag the red fluorescent protein mCherry with ssrA (DAS+4) to measure the degradation rate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None