RNA

Part:BBa_K1223014:Design

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-10-03)
Revision as of 16:10, 3 October 2013 by Schlez (Talk | contribs) (→‎References)


tRNA-Pyl (pylT) gene from Methanosarcina barkeri str. Fusaro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this is a part of the pyrrolysine incorporation machinery.


Source

derived from the genomic sequence of Methanosarcina barkeri. gene name - pylT

clasification: Archaea : Euryarchaeota : Methanomicrobia : Methanosarcinales : Methanosarcinaceae : Methanosarcina

References

Srinivasan,G., James,C.M. and Krzycki,J.A., Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA, Science 296 (5572), 1459-1462 (2002) Milles, S.; Tyagi, S.; Banterle, N.; Koehler, C.; VanDelinder, V.; Plass, T.; Neal, A. P.; Lemke, E. A., Journal of the American Chemical Society 2012, 134 (11), 5187-95.