RNA

Part:BBa_K1223014

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-10-03)
Revision as of 15:39, 3 October 2013 by Registry (Talk | contribs)

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tRNA-Pyl (pylT) gene from Methanosarcina barkeri str. Fusaro

this is the DNA coding sequence for the tRNA of pyrrolysine from the Archaea Methanosarcina barkeri str. Fusaro.

this tRNA is a part of the machinery that is used to incorporate pyrolysine into proteins in E.coli.

the tRNA synthetase that charges the tRNA with pyrrolysine can be found in biobrick BBa_k1223013.

the tRNA has the anticodon CUA - which means that the sense codon needed to incorporate pyrrolysine is TAG - the amber stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
functiontRNA
ligandspyrrolysine, propargyl-lysine
strainMethanosarcina barkeri str. Fusaro