Device

Part:BBa_K1065101

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-06-25)
Revision as of 15:00, 3 October 2013 by Cate (Talk | contribs) (→‎Usage and Biology)

AraCpBAD + BSMT1

The part BBa_K1065100 is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when 5 mM of Arabinose is added to the culture.

This part was cloned by the iGEM UniTN Trento 2013 team for the construction of Wintergreen Devices BBa_K1065102 and BBa_K1065106.


Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1434
    Illegal SapI site found at 961


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Categories
Parameters
None