Composite

Part:BBa_K1149052:Design

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-26)
Revision as of 12:30, 3 October 2013 by Rkelwick (Talk | contribs) (→‎Design Notes)

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Constitutive phaCAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 627
    Illegal BglII site found at 1452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 916
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 1847
    Illegal NgoMIV site found at 1869
    Illegal NgoMIV site found at 2491
    Illegal NgoMIV site found at 2632
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3713


Design Notes

Native promoter and RBS changed to strong constitutive promoter J23104 + B0034

Partial sequence of the constitutive phaCAB construct (BBa_K1149052):

Constitutive_phaCAB.jpg

Source

References