Device

Part:BBa_K1065106

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-08-31)
Revision as of 11:30, 3 October 2013 by Cate (Talk | contribs)

Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase

This is the complete wintergreen odor device from iGEM UniTN-Trento 2013 which encodes for four enzymes: PchB, PchA, BSMT1 and SAM. The device produces methyl salicylate (a fruit ripening inhibitor) and was used for the development of the project B. Fruity by UniTN Trento 2013. This part, containing SAM enzyme BBa_K1065105, is an improvement of part <infopart>BBa_K1065102</partinfo>.

Usage and Biology


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3107
    Illegal BamHI site found at 3856
    Illegal BamHI site found at 4736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
    Illegal AgeI site found at 2942
    Illegal AgeI site found at 5316
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3397
    Illegal SapI site found at 2924


[edit]
Categories
Parameters
None