Part:BBa_K1022114

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1404
  Illegal NheI site found at 1427
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1009
  Illegal AgeI site found at 1079
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1660
  

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]

The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.

Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.

	No cells + 1mM	0.1mM	0.2m	0.3mM	0.4mM	0.5mM	0.6mM	0.7mM	0.8mM	0.9mM	1mM Cell + No IPTG
LB(µL)	90	94	93	92	91	90	89	88	87	86	85	95
Cells(µL)	-	5	5	5	5	5	5	5	5	5	5	5
10X IPTG(µL)	10	1	2	3	4	5	6	7	8	9	10	-