Composite

Part:BBa_K1022114

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-17)
Revision as of 09:32, 3 October 2013 by MaithiliKrishnan (Talk | contribs)

pT7 : Lysis Device

This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1404
    Illegal NheI site found at 1427
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
    Illegal AgeI site found at 1079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1660


Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]

The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.


[1] Part BBa_K1022114


File:Https://static.igem.org/mediawiki/2013/c/c0/114.jpg
450px



[edit]
Categories
Parameters
n/apT7 : Lysis Device