Coding

Part:BBa_K1111012:Experience

Designed by: Sandra Chaudron, Caroline Desmurget and Mareike Apelt   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 22:15, 2 October 2013 by Sandelisa90 (Talk | contribs) (→‎Sequencing)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1111012

This part can be used in any experience with biotin. Biotinylation of biological material is widely used and quick to do. Once biotinylated, the molecule will be able to attach to the streptavidin expressed in bacteria. For us, the material was nanoparticles, that we nicely biotinylated ([http://2013.igem.org/Team:EPF_Lausanne/Nanoparticles nanoparticles protocol and usage])

Sequencing

We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. The sequencing results are shown here : File:Team-EPF-Lausanne ClustalW2 VR col1&2 ISA.pdf

Microscopy

Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix.

User Reviews

UNIQ2eef763e264ca239-partinfo-00000000-QINU UNIQ2eef763e264ca239-partinfo-00000001-QINU