Reporter

Part:BBa_K1041004:Design

Designed by: Lucy Clark   Group: iGEM13_NRP-UEA-Norwich   (2013-10-02)
Revision as of 19:34, 2 October 2013 by Holusac (Talk | contribs) (→‎Design Notes)


AntG Promoter + Gus gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 498
    Illegal NgoMIV site found at 630
    Illegal NgoMIV site found at 1227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 896


Design Notes

Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041001 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick.

Source

This part was designed by our team to aid our project in identifying new strains of Antimycin-Producing Actinomycetes.The 14 known biosynthetic gene clusters contain four operons: antAB, antCDE, antFG and antHIJKLMNO. The antA gene encodes a unique ECF RNA polymerase sigma factor, referred to as σAntA, which has the sole function of regulating antimycin synthesis by activating transcription of the antFG and antHIJKLMNO genes [1]. Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all known gene clusters [2]. Due to this property a biosensor was designed with the AntA-regulated promoter (antGp) controlling the expression of the reporter Gus. This part was produced by performing restriction digests of the part BBa_K1041001.

References