Regulatory

Part:BBa_K1151002:Design

Designed by: Emanuele Rizzo   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 19:09, 2 October 2013 by LeleBiotec (Talk | contribs) (→‎Source)

Double divergent promoter pnikR-pexbB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified by PCR from the plasmid received. 

                                    IntergenicaUnisalento.jpg

Figure 1: PCR results of the intergenic region.


Primers used (including Biobricks' Prefix and Suffix, lowercase):

IntergenicaFor: gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG

pexbrev: gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC

Source

We received this part by Dr. Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the double divergent promoter (from the genome of H. pylori G27) inserted into a pGEM T-Easy plasmid (as pNKTB).

References

1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.