Composite

Part:BBa_K1111005

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 19:09, 2 October 2013 by GigiGoesGugu (Talk | contribs)

Constitutive promoter inducing GFP expression This plasmid contains the constitutive promoter BBa_J23119, that was designed by the 2007 Berkley Team. We inserted it into the biobrick BBa_I746908 instead of the AraC promoter. The cells that contained this construct turned bright green. We used them as a positive control to characterize two of our other parts which contain the same plasmid but have inducible pormoters instead.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


[edit]
Categories
Parameters
n/aconstitutive promoter inducing GFP expression