Regulatory

Part:BBa_K1151002:Design

Designed by: Emanuele Rizzo   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 19:03, 2 October 2013 by LeleBiotec (Talk | contribs) (→‎Design Notes)

Double divergent promoter pnikR-pexbB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified by PCR from the plasmid received. 

                                    IntergenicaUnisalento.jpg

Figure 1: PCR results of the intergenic region.

Primers used (including Biobricks' Prefix and Suffix, lowercase):

IntergenicaFor: gtttcttcgaattcgcggccgcttctagagTGAGAAAAATCCTTTTTTG

pexbrev: gtttcttcctgcagcggccgctactagtaGCACCCTATAAGAAGGCATC

Source

This part comes from H. pylori genomic sequence and it was amplified by PCR.

References

1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.