Part:BBa_K1111002:Experience

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Applications of BBa_K1111002

We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.

Gibson Assembly

We used Gibson Assembly to insert the Cad promoter into the pSB1C3 Backbone. We used the biobrick BBa_I746908 where we remove the Arac promoter but left the superfolded GFP. This way, the latter was under control of our Cad promoter.

Sequencing

Figure 1:

User Reviews

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