Reporter

Part:BBa_K1151011

Designed by: Gian Piero Lazzari   Group: iGEM13_UniSalento_Lecce   (2013-09-23)
Revision as of 12:12, 2 October 2013 by LeleBiotec (Talk | contribs) (→‎PCR with VF2 and VR2 primers)

GFP nickel regulated 3

This is a nickel sensitive system driven by the nikR-exbB intergenic region (BBa_K1151002) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Boiling prep and digestion with restriction enzymes

                   EPEP3.jpg

Figure 1: Digestion result of boiling prep plasmids.

PCR with VF2 and VR2 primers

                PCR77.jpg

Figure 2: PCR results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 919


[edit]
Categories
Parameters
n/aGFP nickel regulated 3