Regulatory

Part:BBa_K1151005

Designed by: Emanuele Rizzo   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 11:11, 2 October 2013 by LeleBiotec (Talk | contribs) (→‎Boiling prep and digestion with restriction enzymes)

pUreA

This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene.

Boiling prep and digestion with restriction enzymes

Once inserted the gene coding for pUreA in a PSB1C3 plasmid (digestion and ligation), we transformed DH5a cells with the construct and then we recovered the ligated plasmid by boiling prep. We then proceeded with the digestion with EcoRI and PstI to check.


Boiling1.jpg

Figure 1:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None