Coding
dxs (B. su

Part:BBa_K1088000:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-07-09)
Revision as of 10:03, 2 October 2013 by Thigers (Talk | contribs) (→‎Design Notes)

1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 1720
    Illegal PstI site found at 1121
    Illegal PstI site found at 1567
    Illegal NgoMIV site found at 1020
    Illegal AgeI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 862
    Illegal SapI.rc site found at 1561


Design Notes

we changed TTG start codon to ATG to improve translation efficiency. We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites

<img src="SDU2013_Future_1.jpg" alt="some_text">

Source

B. subtilis 168

References