Device

Part:BBa_K1150026

Designed by: Freiburg 2013   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 09:30, 2 October 2013 by AnneM (Talk | contribs)

uniCAS Red Light Switch Part II - Activator


uniCAS red Light Switch Part II - Activator
Function Activation domain of red light

induced gene expression control

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Fusion protein of [1] and [2]. This fusion protein PhyB-VP16 is an interaction partner of [3]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [4] protein. \\ The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.)\\ This system enables activation of gene expression induced by red light.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 1076
    Illegal BamHI site found at 1158
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1325


[edit]
Categories
Parameters
None