Part:BBa_K1151000:Design
Nickel-responsive pleiotropic regulator (HpNikR)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was amplified by PCR from the plasmid received by us.
Figure 1: PCR results of nikR cds.
Primers used (including Biobrick Prefix and Suffix, lowercase):
nikRFor: gtttcttcgaattcgcggccgcttctagATGGATACACCCAATAAAGACG
nikRRev: gtttcttcctgcagcggccgctactagtattattaCTATTCATTGTGTTCAAAG
Source
We received this part by Dr. Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the gene encoding NikR (from the genome of H. pylori G27) inserted into a pET-15b plasmid.
References
1. Muller et al., Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori. Nucleic Acids Res. 2011; 39(17):7564-75.
2. Dian et al., Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel -bound States. J Mol Biol. 2006; 25;361(4):715-30.
3. Dosanjh et Michel, Microbial nickel metalloregulation: NikRs for nickel ions. Curr Opin Chem Biol. 2006 (2):123-30.
4. Ernst et al., The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori . Infect Immun. 2005; 73(11):7252-8.
5. Abraham et al., The metal- and DNA-binding activities of Helicobacter pylori NikR. Journal of Inorganic Biochemistry. 100 (2006): 1005–1014.
6. West et al., Holo-Ni(II)HpNikR is an asymmetric tetramer containing two different nickel binding sites. J Am Chem Soc. 2010 October 20; 132(41): 14447–14456.