Device

Part:BBa_K1150026

Designed by: Freiburg 2013   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 09:15, 2 October 2013 by AnneM (Talk | contribs)

uniCAS Red Light Switch Part II - Activator


PhyB
Function Activation domain of red light

induced gene expression control

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Fusion protein of [1] and [2]. This fusion protein PhyB-VP16 is an interaction partner of dCas9-UVR8. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. If the system is exposed to UVB light (311 nm), the UVR8 receptor changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 1076
    Illegal BamHI site found at 1158
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1325


[edit]
Categories
Parameters
None