Device

Part:BBa_K1141002:Design

Designed by: Quentin LUBART   Group: iGEM13_Grenoble-EMSE-LSU   (2013-09-27)
Revision as of 21:20, 1 October 2013 by Simon.pacouret (Talk | contribs) (Corrected design notes)

Plac-RBS-KillerRed (IPTG-inducible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 289
    Illegal BsaI.rc site found at 580


Design Notes

In the original sequence coming from the pQE30 plasmid (see part BBa_K1141001) there was an Ecor1 restriction site between the promoter and the RBS. We removed this site in order to made this biobrick compatible with RFC[10], and 3A assembly.


Source

The KillerRed coding sequence originally came from the ADDGENE plasmid pBabe-KR-CENPA

References