Device

Part:BBa_K1141002

Designed by: Quentin LUBART   Group: iGEM13_Grenoble-EMSE-LSU   (2013-09-27)
Revision as of 21:16, 1 October 2013 by Simon.pacouret (Talk | contribs) (Corrected main description and added info for biological use of the part)

Plac-RBS-KillerRed (IPTG-inducible)

KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI). So the production of KillerRed is IPTG dependent. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 289
    Illegal BsaI.rc site found at 580


[edit]
Categories
Parameters
None