Intermediate

Part:BBa_K1075024

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)
Revision as of 20:47, 1 October 2013 by Leonievb (Talk | contribs)

RBS34- mCherry- (Ec)ssrA(DAS+4)-TT

RBS34-mCherry-ssrA-TT

Usage and Biology

The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB. The tagged protein in this case is mCherry, a red fluorescing protein. Degradation can be measured by the decrease of light intensity. Prefixed is RBS, the binding site for a ribosome and it also includes a double terminator 'TT', which interrupts the translation. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1509
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None