Intermediate

Part:BBa_K1075021

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)
Revision as of 20:34, 1 October 2013 by Leonievb (Talk | contribs)

E. coli ssrA(DAS+4)-TT

ssrA-TT

Usage and Biology

The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB. It includes a double terminator 'TT', which interrupts the translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None