Translational_Unit
Part:BBa_K1223005:Design
Designed by: Orr Schlesinger Group: iGEM13_BGU_Israel (2013-09-07)
cI translational unit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This part was designed to function as a biological timer for our P.A.S.E 1 system. It includes an assembly of two existing parts from the Registry, Lac/Ara-1 IPTG Inducible promoter (BBa_K354000) and LVA-tagged cI repressor protein (BBa_K327018). In order to extend the half life of the cI protein, we removed the LVA tail.
The part was characterized through sequencing and restriction digest with PvuII and HindIII. In addition, the promoter's performance under the presence of IPTG and Arabinose was analyzed via comassie staining. The cI protein is 29 KDa.
lane1: undigested pUC57-cI
lane2: pUC57-cI digested with PvuII and HindIII
lane3: DS5000 ladder
Source
genebank, iGEM parts registry