Generator

Part:BBa_K1216002

Designed by: Parvathi Chandran   Group: iGEM13_ETH_Zurich   (2013-08-29)
Revision as of 12:40, 1 October 2013 by Sabine.oesterle (Talk | contribs) (→‎Usage and Biology)

Acetyl esterase (aes) from Escherichia Coli

Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrolysis of esters of p-nitrophenyl derivatives

3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB]

A form of this protein with added TEV and poly-HIS tags can be found here.

Usage and Biology

The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[1] . Further it can be used for desacteylation of β-Lactam antibiotics[1] .


The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[2] [3].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 951


Michaelis Menten kinetic of the Cell free extract with aes

AES reaction.png
Hydrolase AES.png

References

  1. [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
  2. [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
  3. [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]
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Parameters
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