Composite

Part:BBa_K1132023:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)
Revision as of 12:25, 1 October 2013 by Mesnageclem (Talk | contribs)

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strong prom-strong rbs-rbs-tetR-term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong promoter with strong rbs was assembled to a preexisting biobrick rbs-TetR-term (BBa_P0440) and permits to express TetR in great quantity. The presence of two ribosome binding sites before TetR coding sequence doesn't show any issue to express TetR. The system of TetR-pTet was described there : BBa_K1132022


Source

BBa_K608002
BBa_P0440

References