Composite

Part:BBa_K1132021:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)
Revision as of 12:19, 1 October 2013 by Mesnageclem (Talk | contribs)

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TetR-pTet-rbs-mRFP1-term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
    Illegal AgeI site found at 1595
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is a tool to use the couple TetR-pTet system. In this construct, a modified coding region of TetR (BBa_C0040) was assembled before pTet-mRFP (Bba_I13521). pTet is a constitutive promoter. TetR is an inducer that binds to pTet promoter and thus, represses the expression of the downstream gene controled by pTet. Supply of tetracycline or its analog aTc (anhydrotetracycline) is known to bind to tetR and invert the operation (inhibits expression of mRFP in this case).
A further construct was made by assembling the promoter BBa_J23116 upstream. (Construct available here : BBa_K1132022).



Source

BBa_P0440
BBa_I13521

References